Journal: High-Throughput

Article Title: Characterization of Gene Families Encoding Beta-Lactamases of Gram-Negative Rods Isolated from Ready-to-Eat Vegetables in Mexico City

doi: 10.3390/ht7040036

Figure Lengend Snippet: Gene families encoding beta-lactamases used in the phylogenetic analysis and primers design.

Article Snippet: The metagenomic analysis for the taxonomic classification of bacteria was done by using the software Kraken taxonomic sequence classification system Version 0.10.5-beta (CCB, Johns Hopkins University, Baltimore, MD, USA) [ ].

Techniques: Bla VIM Assay

Journal: High-Throughput

Article Title: Characterization of Gene Families Encoding Beta-Lactamases of Gram-Negative Rods Isolated from Ready-to-Eat Vegetables in Mexico City

doi: 10.3390/ht7040036

Figure Lengend Snippet: Phylogenetic analysis of the gene family that confer beta-lactam resistance: all sequences were downloaded from NCBI GeneBankt and aligned in ClustalW. The phylogenetic trees were edited in FigTree v 1.4.0. All software was running in a Debian (Stretch) machine.

Article Snippet: The metagenomic analysis for the taxonomic classification of bacteria was done by using the software Kraken taxonomic sequence classification system Version 0.10.5-beta (CCB, Johns Hopkins University, Baltimore, MD, USA) [ ].

Techniques: Software

Journal: High-Throughput

Article Title: Characterization of Gene Families Encoding Beta-Lactamases of Gram-Negative Rods Isolated from Ready-to-Eat Vegetables in Mexico City

doi: 10.3390/ht7040036

Figure Lengend Snippet: Primers sequences. Each primer set* was designed to identify all the members of beta-lactamase gene family reported in the GenBank during the 2015 year.

Article Snippet: The metagenomic analysis for the taxonomic classification of bacteria was done by using the software Kraken taxonomic sequence classification system Version 0.10.5-beta (CCB, Johns Hopkins University, Baltimore, MD, USA) [ ].

Techniques: Sequencing, Amplification, Bla VIM Assay

Journal: High-Throughput

Article Title: Characterization of Gene Families Encoding Beta-Lactamases of Gram-Negative Rods Isolated from Ready-to-Eat Vegetables in Mexico City

doi: 10.3390/ht7040036

Figure Lengend Snippet: Frequency of beta-lactamase gene families identified by end point PCR in roads cultured from ready-to-eat vegetables.

Article Snippet: The metagenomic analysis for the taxonomic classification of bacteria was done by using the software Kraken taxonomic sequence classification system Version 0.10.5-beta (CCB, Johns Hopkins University, Baltimore, MD, USA) [ ].

Techniques: Cell Culture, Bla VIM Assay

Journal: High-Throughput

Article Title: Characterization of Gene Families Encoding Beta-Lactamases of Gram-Negative Rods Isolated from Ready-to-Eat Vegetables in Mexico City

doi: 10.3390/ht7040036

Figure Lengend Snippet: End point polymerase chain reaction for the identification of beta-lactamase gene families. 3% agarosa gel electrophoresis showing the positives amplicons for gene families encoding beta-lactamase, MW = marker (100 bp), bla VIM (133 bp), bla SHV (106 and 141), bla P (204 bp), bla IMP (183 and 192 bp), bla CTX (116, 83 or 226 bp), bla CMY (123 or 160 bp), bla DHA (200, 147 or 117), bla TEM (213), bla CFX (177 bp), bla BIL (128 bp), bla KPC (117 bp), bla OXA (114 bp), bla ROB (126 bp), and bla LAP (126 bp).

Article Snippet: The metagenomic analysis for the taxonomic classification of bacteria was done by using the software Kraken taxonomic sequence classification system Version 0.10.5-beta (CCB, Johns Hopkins University, Baltimore, MD, USA) [ ].

Techniques: Polymerase Chain Reaction, Nucleic Acid Electrophoresis, Marker, Bla VIM Assay

Journal: High-Throughput

Article Title: Characterization of Gene Families Encoding Beta-Lactamases of Gram-Negative Rods Isolated from Ready-to-Eat Vegetables in Mexico City

doi: 10.3390/ht7040036

Figure Lengend Snippet: Beta-lactamase gen families identified by end point PCR in roads cultured from ready-to-eat vegetables.

Article Snippet: The metagenomic analysis for the taxonomic classification of bacteria was done by using the software Kraken taxonomic sequence classification system Version 0.10.5-beta (CCB, Johns Hopkins University, Baltimore, MD, USA) [ ].

Techniques: Cell Culture, Bla VIM Assay

Journal: High-Throughput

Article Title: Characterization of Gene Families Encoding Beta-Lactamases of Gram-Negative Rods Isolated from Ready-to-Eat Vegetables in Mexico City

doi: 10.3390/ht7040036

Figure Lengend Snippet: Genotype of the beta-lactama antibiotic resistome identified by next generation sequencing in roads cultured from ready-to-eat vegetables.

Article Snippet: The metagenomic analysis for the taxonomic classification of bacteria was done by using the software Kraken taxonomic sequence classification system Version 0.10.5-beta (CCB, Johns Hopkins University, Baltimore, MD, USA) [ ].

Techniques: Next-Generation Sequencing, Cell Culture